CPSA Europe 2018

Presentation Abstract

Fluidics and Cell Culture: Applications of Flow Open Up New Possibilities for in vitro Experimentation

David Hughes
CN Bio

Symposium Session 6
Thursday, February 8
1:15 pm - 2:45 pm

The culture of cells as monolayers in multi-well plates, in which medium is infrequently changed and drugs are added as a bolus has dominated in vitro experimentation for decades. Fluidics and microfluidics, either alone or in combined with technologies such as three-dimensional cell culture are opening new experimental possibilities. Here the development, commercialisation and search for applications of two complimentary fluidic based technologies, namely MicroMix and microphysiological system (MPS) will be discussed. MicroMix is a precision addition and remove device enabling the recapitulation of pharmacokinetic profiles within a standard multi-well plates. Applications in oncology combination dosing and scheduling will be described. MPS, commonly referred to as organ-on-a-chip, are in vitro cell cultures which combine perfusion, three dimensional scaffolds and mechanical stimulation to closely control the cellular micro-environment. The opportunities and challenges of using MPS, such as CN Bios LiverChip, for applications in drug discovery and development will be explored.